The 30-share barometer, which gained over 475 points during the past two sessions, declined by 44.75 points, or 0.27 per cent, to 16,438.70 in the first five minutes of trade.
The wide-based National stock Exchange Nifty index followed suit, shedding 11.35 points, or 0.23 per cent, to 4,925.50.
Metal, oil and gas and realty stocks led the fall. Brokers said market participants were seen booking profits after the steep rise over the past two trading sessions.
The selling pressure was exacerbated by a weakening trend in the Asia region, they said.
Meanwhile, in the Asia region, Hong Kong's Hang Seng index was down by 0.37 per cent in morning trade.
In the US, the Dow Jones Industrial Average ended 0.21 per cent lower yesterday.